摘要 |
<p>The invention relates to a process for preparing L-aspartic acid meant for food and pharmaceutical industry. According to the invention, the process consists in that it comprises the inoculum preparation stage, by culturing the stem on a medium containing corn extract and mineral salts, for 18...20 hours, at a temperature of 30...32 C, the bioprocess stage for culture growing on a similar medium but enriched in corn extract, for 22...24 hours, at the temperature of 30...32 C, the biomass separation by centrifuging or filtration, a biomass with an enzymatic activity of 2000...2500 Ui/moistured g being obtained after the admixture of the flocculating agent CS2 0.08...0.1 ml%, the bioconversion of the fumaric acid into L-aspartic acid by using the aspartase enzyme, with an efficiency of at least 95%.</p> |