发明名称 |
Use of isogenic human cancer cells for high-throughput screening and drug discovery |
摘要 |
A strategy for drug-screening is based on cells that are isogenic except for a gene of interest. Each cell can be tranfected with a vector that encodes a different fluorescent protein that can be differentially detected to monitor cell growth. Co-culture of both cells allows facile screening for compounds with selective toxicity towards a gene of interest. The drug screening is broadly applicable for mining therapeutic agents targeted to specific genetic alterations responsible for cancer development.
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申请公布号 |
US2005239051(A1) |
申请公布日期 |
2005.10.27 |
申请号 |
US20050159229 |
申请日期 |
2005.06.23 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
TORRANCE CHRISTOPHER J.;VOGELSTEIN BERT;KINZLER KENNETH W. |
分类号 |
A61K31/41;A61K31/7068;A61P35/00;A61P43/00;C07D257/04;C07H19/06;C07H19/16;C12N5/10;C12N15/09;C12Q1/02;G01N33/15;G01N33/50;(IPC1-7):C12Q1/00;C12Q1/68;C12N5/08 |
主分类号 |
A61K31/41 |
代理机构 |
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代理人 |
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地址 |
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