REVERSIBLY IMMORTALIZED MAMMALIAN LIVER CELLS AND USE THEREOF

摘要

Reversibly immortalized mammalian liver cell lines, in particular, CYNK-1 (accession no. FERM BP-08657, deposited date: 10 March, 2004 (10.03.04), deposited with International Patent Organism Depositary (IPOD), National Institute of Advanced Industrial Science and Technology (AIST), Chuo 6, Higashi 1-1-1, Tsukuba-shi, Ibaraki-ken, 305-8566 JAPAN) which contains an immortalizing gene located between a pair of site-specific recombination sequences and having a suicide gene at a site excluding the above-described pair of site-specific recombination sequences, characterized in that the suicide gene can exhibit its function after cutting off the pair of site-specific recombination sequences, or successive lines thereof; mammalian liver cells obtained by eliminating the immortalizing gene from the immortalized mammalian liver cells line or successive lines thereof; and use of these cells. By using the immortalized mammalian liver cell lines as described above, liver cells in a required number can be surely obtained and used as materials for artificial liver reactors and cell preparations.