发明名称 |
SELECTION MARKER GENE FREE RECOMBINANT STRAINS, METHOD FOR OBTAINING THEM AND USE OF THESE STRAINS |
摘要 |
PROBLEM TO BE SOLVED: To provide a transformed microorganism which comprises only a desired gene, a fragment thereof or modifications in a gene and further comprises as few other DNA used for cloning as possible or no DNA. SOLUTION: Disclosed is a recombinant strain except fungi, wherein the recombinant strain is characterized in that an acetoamidase gene is deleted after the recombinant strain is transformed using the acetoamidase as a selection marker gene. COPYRIGHT: (C)2006,JPO&NCIPI
|
申请公布号 |
JP2005296019(A) |
申请公布日期 |
2005.10.27 |
申请号 |
JP20050166041 |
申请日期 |
2005.06.06 |
申请人 |
DSM NV |
发明人 |
SELTEN GERARDUS CORNELIUS MARIA;SWINKELS BART WILLEM;VAN GORCOM ROBERTUS FRANCISCUS MARIA |
分类号 |
C12N15/09;C12N;C12N1/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/00;C12N15/01;C12N15/31;C12N15/52;C12N15/63;C12N15/64;C12N15/65;C12N15/70;C12N15/73;C12N15/74;C12N15/75;C12N15/80;C12N15/81;C12R1/10;C12R1/125;C12R1/19;C12R1/645;C12R1/66;C12R1/685;C12R1/80;C12R1/865;C12R1/885;(IPC1-7):C12N1/21 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|