PREPARATION FOR SYNTHESIZING CELL-FREE PROTEIN, METHOD FOR SYNTHESIZING PROTEIN USING SAID PREPARATION AND APPARATUS THEREFOR

摘要

The invention relates to a preparation for synthesizing a cell-free protein a method for synthesizing a cell-free protein and an apparatus therefor. The preparation for synthesizing a cell-free protein comprises a cell extract obtained from plant embryo from which completely removed any endosperm contaminants. The preparation is stable and maintains biological functions of said cell extract at room temperature. A means for continuously synthesizing a cell-free protein involves separation of synthesized protein and characterized in that the cell-free protein synthesis is performed during passing material substances through a carrier capable of being molecular sieved as a mobile phase. A means for continuously synthesizing a cell-free protein involving a procedure for introducing into a system a synthesis substrate and/or an energy source, and/or mRNA and/or energy reproduction system enzyme, and involving discharging metabolites from the system immediately in the process of the protein synthesis.