The present invention relates to the field of immunology and human medicine, in particular to the specific active immunotherapy of malignant tumors that depend of TGFalpha for its growth and to the treatment of other TGF-alpha depend diseases by using a vaccine preparation able to provoke an immune-castration of self-TGFalpha. The invention also relates to obtain a vaccine composition containing a combination of TGFalpha with other EGF-R ligands, such as epidermal growth factor (EGF), able to inhibit the proliferation of tumors whose progression depends on these growth factors. In that way would be avoided the resistance generated by tumors vaccines containing each molecule.
