SINGLE MOLECULE AMPLIFICATION AND DETECTION OF DNA
摘要
Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.
申请公布号
EP1587940(A1)
申请公布日期
2005.10.26
申请号
EP20030812089
申请日期
2003.12.19
申请人
CALIPER LIFE SCIENCES, INC.
发明人
KNAPP, MICHAEL, R.;BAKER, JILL, M.;CHOW, ANDREA;KOPF-SILL, ANNE;SPAID, MICHAEL