| 摘要 |
In accordance with the present invention, there is provided a novel restriction endonuclease obtainable from Bacillus thermoglucosidasius 36A (NEB#1384), hereinafter referred to as "BtgZI", which endonuclease: (1) recognizes the nucleotide sequence 5'-GCGATG-3' in a double-stranded DNA molecule as shown below, <tables id="TABLE-US-00001" num="1"> <table frame="none" colsep="0" rowsep="0" tabstyle="monospace"> <tgroup align="left" colsep="0" rowsep="0" cols="4"> <colspec colname="OFFSET" colwidth="14PT" align="left"/> <colspec colname="1" colwidth="133PT" align="left"/> <colspec colname="2" colwidth="70PT" align="left"/> <colspec colname="3" colwidth="0PT" align="left"/> <TBODY VALIGN="TOP"> <ROW> <ENTRY/> <ENTRY/> </ROW> <ROW> <ENTRY/> <ENTRY>5'-GCGATGNNNNNNNNNN↓-3'</ENTRY> <ENTRY>(SEQ ID NO:9)</ENTRY> <ENTRY/> </ROW> <ROW> <ENTRY/> <ENTRY> </ENTRY> </ROW> <ROW> <ENTRY/> <ENTRY>3'-CGCTACNNNNNNNNNNNNNN↑-5'</ENTRY> </ROW> </TBODY> </TGROUP> </TABLE> </TABLES> (wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T); (2) cleaves said sequence in the phosphodiester bonds between the 10th and the 11th nucleotides 3' to the recognition sequence in the 5'-GCGATG-3 strand of the DNA, and between the 14th and 15th nucleotides 5' to the recognition sequence in the complement stand, 5'-CATCGC-3', to produce a 4 base 5' extension; and (3) cleaves double-stranded pBR322 DNA to produce 3 fragments of 2892, 1181 and 288 base pairs.
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