发明名称 |
LIGAND-DEPENDENT PROTEIN SPLICING |
摘要 |
Ligand-dependent inteins allow for modulation of a protein's activity in vivo. Upon binding of the ligand to the ligand-dependent intein inserted into the protein of interest, the hybrid protein undergoes protein splicing removing the intein. The activity of the spliced protein is then restored. A 4-hydroxytamoxifen-dependent intein based on the M. tuberculosis RecA intein is prepared and demonstrated in a variety of exteins contexts. The invention provides a system for engineering other ligand-dependent inteins and using them, including the ligand-dependent inteins themselves, hybrid proteins with the inserted ligand-dependent inteins, polynucleotides encoding inteins and hybrid proteins, and engineered cells. Kits with the materials and reagents necessary for preparing and using ligand-dependent inteins are also included. |
申请公布号 |
WO2005098043(A2) |
申请公布日期 |
2005.10.20 |
申请号 |
WO2005US10805 |
申请日期 |
2005.03.30 |
申请人 |
THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE;LIU, DAVID, R.;BUSKIRK, ALLEN, R. |
发明人 |
LIU, DAVID, R.;BUSKIRK, ALLEN, R. |
分类号 |
C07H21/04;C07K14/35;C07K14/395;C07K14/72;C12N9/14;C12N15/62;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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