CANCER THERAPY VIA THE INHIBITION OF Skp-2 EXPRESSION

摘要

It is intended to provide a double-stranded RNA (siRNA) capable of inhibiting the expression of Skp-2 gene; a double-stranded RNA expression cassette capable of expressing the double-stranded RNA; a double-stranded RNA expression vector containing the double-stranded RNA expression cassette; and a highly safe remedy and a therapy method specific for cancer such as lung small cell carcinoma targeting Skp-2 molecule. A plural number of RNAi target sequence sites are set in the Skp-2mRNA protein translation region and expressed siRNA (i.e., dsRNA showing an RNAi effect) is constructed on a lentivirus vector, thereby give a recombinant virus vector. Then various lung small cell carcinoma cell strains and lung small cell carcinoma are infected with this virus vector so as to confirm its cell growth inhibitory effect in vitro and its growth inhibitory effect in vivo.