MODIFIED POLYNUCLEOTIDES FOR REDUCING OFF-TARGET EFFECTS IN RNA INTERFERENCE

摘要

Methods and compositions for performing RNA interference with decreased off- target effects are provided. The methods and compositions permit effective a nd efficient applications of RNA interference to applications such as diagnosti cs and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2'-O-alkyl or mismatch modification(s) at specific positions on the sense and/or antisense strands.