摘要 |
The present invention provides a method of identifying an enzyme, the method generally involving contacting a sample containing an enzyme with a selected enzyme substrate, where the contacting provides for covalent binding of the substrate to an amino acid of the enzyme to form a covalently modified enzyme; and determining the amino acid sequence of at least a portion of the covalently modified enzyme, using any available peptide sequencing technology, such as tandem mass spectrometry. The present invention further provides methods of identifying a nucleic acid encoding an enzyme, the methods generally involving identifying an enzyme; and, based on the amino acid sequence of at least a portion of the enzyme, designing nucleic acid probes or primers that hybridize to the nucleic acid encoding the enzyme. |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;NEWMAN, JACK, D.;RENNINGER, NEIL;MARTIN, VINCENT J.J.;KEASLING, JAY, D.;REILING, KEITH, KINKEAD |
发明人 |
NEWMAN, JACK, D.;RENNINGER, NEIL;MARTIN, VINCENT J.J.;KEASLING, JAY, D.;REILING, KEITH, KINKEAD |