SYSTEM AND METHOD FOR MULTIPLE LASER TRIGGERING

摘要

A system for measuring the irradiance of a fluorescently labeled particle (38) having a cytometric flow chamber; a plurality of excitation light sources (32); a plurality of scatter detectors, each configured to detect light from only one of the plurality of excitation light sources (32) and arranged so as to detect scattered light from the particle (38); a trigger (60) connected to the plurality of scatter detectors, the trigger (60) emitting a signal when scattered light incident on one of the scatter detectors is exceeding a predetermined threshold value; collection optics (120); at least one fluorescence detector to receive emissions collected by the collection optics (120) and generate an output, the at least one fluorescence detector being configured to respond only to a discrete number of wavelength bands; and an integrator (118) for recording the output of the at least one fluorescence detector in response to a signal from the trigger (60).