发明名称 |
FUSED PROTEIN ELICITING BIOLOGICAL ACTIVITY OF INTERFERON-ALPHA, DIMERIC FUSED PROTEIN, PHARMACEUTICAL COMPOSITION COMPRISING THEREOF, DNA MOLECULE (VARIANTS) AND METHOD FOR TARGETING INTERFERON-ALPHA INTO LIVER TISSUE |
摘要 |
FIELD: genetic engineering, proteins, medicine, pharmacy. ^ SUBSTANCE: invention relates to a method for preparing a fused protein representing immunoglobulin Fc-fragment and interferon-alpha and can be used in treatment of hepatitis. Method involves construction of a fused protein comprising immunoglobulin Fc-fragment prepared from Ig G1 or Ig G3 in direction from N-end to C-end and the end protein comprising at least one interferon-alpha. Fc-fragment and the end protein are joined directly or by a polypeptide bridge. The fused protein is used for preparing a pharmaceutical composition used in treatment of liver diseases and in a method for targeting interferon-alpha into liver tissues. Invention provides preparing the fused protein eliciting with biological activity of interferon-alpha providing its concentrating in liver and showing enhanced solubility, prolonged half-time life in serum blood and enhanced binding with specific receptors. ^ EFFECT: improved targeting method, valuable biological properties of fused protein. ^ 10 cl, 5 dwg, 9 ex |
申请公布号 |
RU2262510(C2) |
申请公布日期 |
2005.10.20 |
申请号 |
RU20010130984 |
申请日期 |
2000.05.19 |
申请人 |
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发明人 |
SUN JAPING;DZHILLIZ STEFEN D.;LO KIN-MING |
分类号 |
C12N15/09;A61K35/76;A61K38/00;A61K38/11;A61K38/21;A61K48/00;A61P1/16;A61P31/20;A61P43/00;C07K14/52;C07K14/56;C07K14/715;C07K16/18;C07K19/00;C12N5/10;C12N15/21;C12N15/62;C12N15/63;C12N15/86;C12N15/863;C12P21/02;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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