发明名称 |
Methods for in situ generation of nucleic acid arrays |
摘要 |
Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group generation step. A feature of the subject methods is that the functional group generation step is performed by sequentially flowing a plurality of different fluids across the surface of a substrate (110) in a manner such that the surface is not contacted with a with a triple phase interface line. Also provided are the arrays (112) produced using the subject methods, as well as methods for use of the arrays and kits that include the same. <IMAGE>
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申请公布号 |
EP1586374(A2) |
申请公布日期 |
2005.10.19 |
申请号 |
EP20050251938 |
申请日期 |
2005.03.29 |
申请人 |
AGILENT TECHNOLOGIES, INC. |
发明人 |
PECK, BILL, J.;LEPROUST, ERIC, M. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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