发明名称 |
NON-HYPERTENSIVE PEGYLATED HEMOGLOBINS AND METHODS OF PREPARATION THEREOF |
摘要 |
The present invention provides a hemoglobin molecule (Hb) having six ± one PEG chains, wherein two of said PEG chains are bound to Cys-93 (beta) of Hb, and the remaining PEG chains are bound to thiol groups introduced on epsilon-NH2 of Hb. The present invention also provides a process for preparing a modified hemoglobin molecule (Hb), comprising the steps of: (a) reacting Hb with 8-15 fold excess of iminothiolane to form thiolated Hb; and (b) reacting the thiolated Hb with 16-30 fold excess of PEG functionalized with a maleimide moiety, to form the modified Hb. |
申请公布号 |
EP1585538(A1) |
申请公布日期 |
2005.10.19 |
申请号 |
EP20030799982 |
申请日期 |
2003.12.18 |
申请人 |
ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY |
发明人 |
ACHARYA, SEETHARAMA, A.;MANJULA, BELUR, N. |
分类号 |
A61K38/16;A61K38/00;A61K38/42;A61K47/10;C07K14/805;(IPC1-7):A61K38/16 |
主分类号 |
A61K38/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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