| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for preparing a high-purity DNA with its molecular weight made even and its double strand retained, and to provide the DNA as a functional material. SOLUTION: The method for preparing the DNA as a functional material comprises the following procedure: A high-purity macromolecular DNA is subjected to enzymolytic treatment with an enzyme capable of breaking a DNA strand to effect breakage adjustment to a DNA with an aimed molecular weight, the resultant DNA is further subjected to enzymolytic treatment with an enzyme capable of specifically breaking a single-stranded DNA to break the single-stranded DNA, and by removing the thus broked single-stranded DNA, the objective DNA with its molecular weight made even to an aimed level and increased proportion of double-stranded DNA is obtained. A method for preparing a highly functional DNA film is also provided, comprising the following procedure: A solution of the DNA obtained above and a lipid solution are mixed together under agitation to form a DNA-lipid complex, which is then hot-pressed or dissolved in an organic solvent and spread widely and dried to obtain the objective thin DNA film. COPYRIGHT: (C)2006,JPO&NCIPI
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