| 摘要 |
Conjoined polynucleotides are linked RNA and/or DNA that comprise at least tow catalytic domains which function in concert to provide a chemical transformation involving multiple sequential or component reactions. In many embodiments, the domains are fused together, typically by means of conventional 3'->5' phosphodiester bonds, with or without intervening nucleotides which are not part of the catalytic domains per se, to form conjoined polynucleotides using standard ligation procedures. Conjoined DNA comprising kinase and adenylase domains and conjoined polynucleotides comprising kinase; adenylase, and ligase domains are useful in DNA cloning.
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