ARTIFICIAL ACTINOMYCES H<+>-PYROPHOSPHATASE GENE AND SYSTEM FOR EXPRESSING THE SAME IN ESCHERICHIA COLI

摘要

It is intended to provide an artificial actinomyces H<+>-PPase gene derived from wild type actinomyces H<+>-PPase gene by partial modification of the base sequence thereof to thereby construct an expression system of Escherichia coli having the actinomyces H<+>-PPase gene integrating therein and provide a method of efficiently producing actinomyces H<+>-PPase. An artificial actinomyces H<+>-PPase gene derived from the sequence of wild type actinomyces H<+>-PPase gene by substituting a rare codon for of Escherichia coli by another codon in such a manner as not changing the amino acid sequence encoded thereby. The thus modified artificial actinomyces H<+>-PPase gene can stably exist on a plasmid in of Escherichia coli and its transformant can similarly grow to Escherichia coli. The above-described artificial actinomyces H<+>-PPase gene enables mass production of actinomyces H<+>-PPase that has been impossible hitherto.