| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a PCR (polymerase chain reaction) equipment being an optical equipment for quantitative monitoring of replicative DNA which is improved in a dynamic range, capable of automatically selecting the exposure time for expanding a dynamic range, capable of automatic drift adjustment, easy to operate, relatively low price, and easy to change the optical system for storing a different fluorescence dye. <P>SOLUTION: The equipment includes a light source, a light guide means for guiding a light beam, an optical detector, and a means for processing a data signal. The light source emits source beam containing at least one frequency of primary excitation frequency capable of making fluorescence emit with emission frequency. A 1st means is so arranged as to activate an excitation beam of excitation frequency while receiving the source beam. A 1st focus means is arranged to focus the excitation beam in each suspension to make the primary dye emit emission beams with intensity indicating the emission frequency and the DNA concentration of each suspension. A focus means receives an emission beam and makes it pass through. A 2nd means is arranged to receive the emission beam to pass through the emission beam of emission frequency to the other focus means which focuses the emission beam on the detector. The detector generates a primary data signal representing the emission beam and generates the concentration corresponding to the DNA of each small bottle. The processor displays the concentration of DNA by acquiring the primary data signal. <P>COPYRIGHT: (C)2006,JPO&NCIPI |
