发明名称 |
Rapid method of detection and enumeration of sulfide-producing bacteria in food products |
摘要 |
A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A growth medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. In one embodiment, a plurality of fluorescence measurements are taken during an incubation period of about 3 hours to 17 hours at 30° C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The time to detection of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. In another embodiment, a visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective after about 3 hours to 17 hours of incubation.
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申请公布号 |
US2005221419(A1) |
申请公布日期 |
2005.10.06 |
申请号 |
US20050133084 |
申请日期 |
2005.05.19 |
申请人 |
LORENTZEN GRETE;SKJERDAL OLAUG T;BERG JAMES D;SKJERDAL OLAUG T;LORAITZAR GROA |
发明人 |
LORENTZEN GRETE;SKJERDAL OLAUG T.;BERG JAMES D.;SKJERDAL OLAUG T.;LORAITZAR GROA |
分类号 |
C12Q1/04;C12Q1/06;C12Q1/08;(IPC1-7):C12Q1/04 |
主分类号 |
C12Q1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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