发明名称 |
High throughput screening method for biological agents affecting fatty acid biosynthesis |
摘要 |
Provided is a screening method for compounds affecting fatty acid biosynthesis, the method comprising: providing a reaction mixture comprising: an acyl carrier moiety or enzymes and precursors sufficient to generate the acyl carrier moiety; a bacterial enzymatic pathway comprising at least two consecutively acting enzymes selected from the group consisting of: malonyl-CoA:ACP transacylase, beta-ketoacyl-ACP synthase III, NADPH dependent beta-ketoacyl-ACP reductase, beta-hydroxylacyl-ACP dehydrase and enoyl-ACP reductase; and substrates and cofactors required for the operation of the enzymes; contacting the reaction mixture with a prospective bioactive agent; conducting a high throughput measurement of the activity of the enzymatic pathway; and determining if the contacting altered the activity of the enzymatic pathway.
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申请公布号 |
US6951729(B1) |
申请公布日期 |
2005.10.04 |
申请号 |
US20020089019 |
申请日期 |
2002.03.25 |
申请人 |
AFFINIUM PHARMACEUTICALS, INC. |
发明人 |
DEWOLF, JR. WALTER E;KALLENDER HOWARD;LONSDALE JOHN T |
分类号 |
C12Q1/48;C12Q1/61;(IPC1-7):C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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