Vaccine composition

摘要

Disclosed is a genetically-engineered bleb preparation isolated from a modified Neisseria meningitidis strain characterized in that said preparation is obtained by employing the following process: a) a process of reducing immunodominant variable or non-protective antigens within the bleb preparation comprising the steps of determining the identity of such antigen, engineering a bacterial strain to produce less or none of said antigen, and making blebs from said strain, wherein one or more of said antigens are selected from a list consisting of: PorA, PiIC, ThpA, TbpB, LbpA, and LbpB; and, optionally employing one or more further processes selected from the group b) to i) which are disclosed in the specification. Further disclosed is the use of the bleb preparation in the manufacture of a medicament for immunising a human against a disease caused by Neisseria meningitides.