PROTEIN AND PEPTIDE LIGATION PROCESSES AND ONE-STEP PURIFICATION PROCESSES

摘要

Provided are enzyme-catalyzed methods, and related products and kits, for efficiently linking a molecule to a protein or peptide and conjugates generated therefrom. An enzyme often utilized has transamidase activity, such as a sortase or related enzyme. Provided also are products, processes and kits for purifying in one step a recombinant target protein or target peptide initially expressed as a fusion product. The fusion product includes a solid phase association region, a peptidase, a peptidase recognition sequence and a target protein or target peptide. The peptidase is capable of cleaving the fusion protein when the fusion is bound to a solid support that specifically binds the solid phase association region, whereby the target protein or target peptide is released from the solid support in a substantially purified form. The peptidase sometimes has hydrolase activity, such as a sortase or related enzyme for example.