ГЕН ГЛИКОПРОТЕИНА (G-ГЕН) РОССИЙСКОГО РЕФЕРЕНСНОГО ШТАММА ЗЛ-4 ВИРУСА ВЕСЕННЕЙ ВИРЕМИИ КАРПА И РЕКОМБИНАНТНЫЕ ПЛАЗМИДНЫЕ ДНК PCDNA-G И PBACARPVAX-G, ЭКСПРЕССИРУЮЩИЕ ГЕН ГЛИКОПРОТЕИНА И ОБЕСПЕЧИВАЮЩИЕ РАЗВИТИЕ ЗАЩИТНОГО ИММУНИТЕТА У РЫБ ПРОТИВ ЗАРАЖЕНИЯ ВИРУСОМ ВЕСЕННЕЙ ВИРЕМИИ КАРПА

摘要

FIELD: genetic engineering, biochemistry, pisciculture. ^ SUBSTANCE: invention relates to the determined nucleotide sequence encoding glycoprotein of the Russian isolate ZL-4. Based on this sequence DNA-plasmids are constructed that provide expression of glycoprotein of the Russian isolate ZL-4 in fishes. DNA-vaccine constructed on basis of indicated DNA-plasmids provides the development of protective immunity in fishes against the following infection with ZL-4 isolate of carp spring viremia virus. Using the invention provides protection of carp in Russian aquaculture from the following infection with carp spring viremia virus. ^ EFFECT: valuable properties plasmid DNA and glycoprotein gene. ^ 3 cl, 4 dwg, 3 ex