Method and apparatus for determination of RNAi cell transfection effects by multiple gene expression analysis on micro-arrays

摘要

The invention provides a tool and a method for the easy interpretation of the changes occurring in a cell, being a three dimensional complex and control system when transfected by RNAi. The method is based on the analysis of a limited number of data obtained by quantifying the intensity of the signals present on spots distributed in a two dimensional surface. The invention allows to observe the effects of the presence of a particular RNAI on the cells main vital cellular functions together with the possible side or deleterious effects resulting from the transfection process or to the presence of the RNAi in a cell. The invention also provides a method for the determination of change in the three dimensional status of a cell, wherein an array containing nucleic acids or proteins belonging to or being representative for at least 5 vital cellular functions together with 3 deleterious functions