| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for preparing a cell culture element capable of transplanting and imbedding into an affected part for relieving patients with Parkinson's disease, Alzheimer's syndrome, diabetes mellitus, osteomalacia by substituting only a light-exposed part of a porous fluororesin inner hole wall with a hydrophilic group by photochemical reaction of ultraviolet light to prepare a base for three-dimensional cell culture and culturing and proliferating a cell such as a dopamine-producing cell or a fibroblast, an osteoblast, a collagen-producing cell, a stem cell, a pulpous nucleus cell or an insulin-producing cell in the fine inner hole wall as a three dimensional structure. <P>SOLUTION: An alcohol is permeated into a porous part of a plastic such as fluororesin or water is made attachable into porous inner holes of the plastic by carrying out electric discharge in order to make the porous inner holes such as of fluororesin hydrophilic. When water is charged into the inner holes and water is dropped from the above and a quartz glass window is covered on the inner holes and the glass window is irradiated with ArF laser having 193 nm wavelength, a cell chip in which the surface and the inner holes are hydrophilic is prepared. The cell chip is sterilized with a gas and cell culture is carried out to produce the cell chip for transportation to human bodies. <P>COPYRIGHT: (C)2005,JPO&NCIPI</p> |
