摘要 |
The present invention is based, in part, on the discovery that cell growth, cell viability, and antibody production in a membrane system such as hollow fiber system can be enhanced by culturing cells in the presence of serum on the cell side and in a medium containing ethanolamine in the absence of serum on the non-cell side. Accordingly, the present invention provides methods for the enhanced growth of cells while minimizing the use of serum. |