HEAT-RESISTANT FIREFLY LUCIFERASE, HEAT-RESISTANT FIREFLY LUCIFERASE GENE, NEW RECOMBINANT DNA AND METHOD FOR PRODUCING HEAT-RESISTANT FIREFLY LUCIFERASE

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a heat-resistant firefly luciferase by changing specific amino acid residues in a mild type firefly luciferase into hydrophobic amino acid residues.

SOLUTION: This heat-resistant firefly luciferase is characterized by changing the amino acids at the 217- and 315-positions in the amino acid sequence of the mild type firefly luciferase or the amino acids at the positions equivalent to the 217- and 315-positions in the amino acid sequence of Luciola cruciata or Luciola lateralis luciferase into hydrophobic acid residues. The heat-resistant firefly luciferase gene. A vector integrated with the gene. The method for producing the heat-resistant firefly luciferase comprises using the vector to produce the heat-resistant firefly luciferase. The heat-resistant firefly luciferase can efficiently be obtained by the method, and is highly useful on industry.

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