发明名称 |
ASSAY FOR IDENTIFICATION OF BIOACTIVE COMPOUNDS THAT INTERACT WITH HEAT SHOCK PROTEIN 90 |
摘要 |
A method for evaluation of molecules to identify those that can act as therapeutic inhibitors of Hsp90 makes use of a fluorescence polarization (FP) assay and a cell based assay, either individually or in combination. The FP assay uses a fluorescently-labeled Hsp90 binding agent and measure the degree of fluorescence polarization relative to the standard. A decrease in the degree of polarization indicates that fluorescently-labeled Hsp90 binding agent has been wholly or partially displaced by a candidate molecule, and identifies the molecule as having activity as an inhibitor of Hsp90. The cell based assay tests for decrease is an Hsp90-dependent activity of normal or tumor cells. |
申请公布号 |
WO2005012482(A3) |
申请公布日期 |
2005.09.15 |
申请号 |
WO2004US21297 |
申请日期 |
2004.06.30 |
申请人 |
SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH;CHIOSIS, GABRIELA;ROSEN, NEAL;HUEZO, HENRI |
发明人 |
CHIOSIS, GABRIELA;ROSEN, NEAL;HUEZO, HENRI |
分类号 |
C12N;G01N21/21;G01N33/52;G01N33/53;G01N33/566;G01N33/567 |
主分类号 |
C12N |
代理机构 |
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代理人 |
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地址 |
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