| 摘要 |
FIELD: veterinary medicine. ^ SUBSTANCE: vaccine has avirulent antigen material produced from O112-DEP strain homological virus, protection medium and adjuvant taken in effective proportions. The strain is deposited in micro-organism VGNKI collection under registry number of O112-DEP. The O112-DEP strain virus is reproduceable in 9-11 days old SPF-hen embryos having infectious activity of at least 6.9 lg EID50/cm3. The vaccine comprises ethylene imine dimer in 0.1% concentration as inactivation agent. The antigen material is mixed with protection medium after activation and subjected to lyophilization. Before being used, the dried antigen material is solved in adjuvant containing aerosyl, saponin, glycerol and phosphate buffer solution taken in effective proportions. The vaccine is intramuscularly introduced for immunizing clinically healthy 25-30 days old birds in the amount of 0.5 cm3. Immunity comes to a vaccinated bird at the fourteenth-twenty first day after vaccination. ^ EFFECT: high antigenic and immunogenic activity; no adverse side effects. ^ 8 cl, 5 tbl
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