ПРОСТОЙ, ЭФФЕКТИВНЫЙ И БЫСТРЫЙ СПОСОБ ФЕРМЕНТАТИВНОЙ МОДИФИКАЦИИ И СИНТЕЗА НУКЛЕИНОВОЙ КИСЛОТЫ IN VITRO С ИСПОЛЬЗОВАНИЕМ МИКРОВОЛНОВОГО ИЗЛУЧЕНИЯ

摘要

FIELD: biotechnology, in particular enzymatic reactions using nucleic acids as substrate. ^ SUBSTANCE: enzymatic treatment of nucleic acid is carried out with one or two enzymes in presence of microwave irradiation, buffer and other ingredients, being necessary for enzyme reaction. ^ EFFECT: simple, effective and fast method for enzymatic modification and synthesis of nucleic acid in vitro. ^ 13 cl, 7 dwg, 1 tbl, 10 ex