发明名称 |
Dual phase multiplex polymerase chain reaction |
摘要 |
Highly specific and sensitive methods were developed for multiplex amplification of nucleic acids on supports such as microarrays. Based on a specific primer design, methods include five types of amplification that proceed in a reaction chamber simultaneously. These relate to four types of multiplex amplification of a target DNA on a solid support, directed by forward and reverse complex primers immobilized to the support and a fifth type-pseudo-monoplex polymerase chain reaction (PCR) of multiple targets in solution, directed by a single pair of unbound universal primers. The addition of the universal primers in the reaction mixture increases the yield over the traditional "bridge" amplification on a solid support by approximately ten times. Methods that provide multitarget amplification and detection of as little as 0.45-4.5x10<SUP>-12 </SUP>g (equivalent to 10<SUP>2</SUP>-10<SUP>3 </SUP>genomes) of a bacterial genomic DNA are disclosed.
|
申请公布号 |
US2005196760(A1) |
申请公布日期 |
2005.09.08 |
申请号 |
US20040794381 |
申请日期 |
2004.03.05 |
申请人 |
PEMOV ALEXANDER;BAVYKIN SERGEI |
发明人 |
PEMOV ALEXANDER;BAVYKIN SERGEI |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|