发明名称 |
New recombinant microorganisms comprising increased concentration of D-amino acid oxidase, L-amino acid dehydrogenase, co-substrate NADH enzyme and catalase than the parental microorganism, useful to prepare L-amino acids from D-amino acids |
摘要 |
Recombinant microorganisms (I) (comprising a increased concentration of or activity of D-amino acid oxidase, L-amino acid dehydrogenase, an enzyme regenerating the co-substrate NADH and optionally catalase than the parental microorganism) is new. Independent claims are also included for: (1) a vector (A) comprising one or more of D-amino acid oxidase and amino acid dehydrogenase, for regenerating co-substrate NADH enzyme-coding nucleotide sequences; and (2) preparation of L-amino acids from D-amino acids using a enantioselective enzymatic synthesis, comprising adding (I) (comprising increased concentration of or activity of D-amino acid oxidase, L-amino acid dehydrogenase, an enzyme regenerating the co-substrate NADH and optionally catalase than the parental microorganism (e.g. wild type)) in solution containing the D-amino acids to convert the D-amino acids into L-amino acids; and isolating the formed L-amino acids. |
申请公布号 |
DE102004008445(A1) |
申请公布日期 |
2005.09.08 |
申请号 |
DE20041008445 |
申请日期 |
2004.02.19 |
申请人 |
DEGUSSA AG |
发明人 |
HUMMEL, WERNER;GEUEKE, BIRGIT;OSWALD, STEFFEN;WECKBECKER, CHRISTOPH;HUTHMACHER, KLAUS |
分类号 |
C12N9/06;C12N9/08;C12N15/52;C12P13/04;C12P13/06;C12P13/12 |
主分类号 |
C12N9/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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