| 摘要 |
The present invention relates to biopolymer detection methods based on antigen-antibody interactions, wherein the methods show improved S/N ratios, improved detection sensitivities, and reduced detection times. The present invention also relates to application of these methods to biochips. The invention further relates to antibody fixation methods wherein antibody molecules are immobilized to amide group-containing gels, or amide group-containing gels on insoluble substances. Using such gels prevents the nonspecific adsorption of antibody-binding molecules. By embedding these antibody-binding molecules in such gels, their antibody-binding activity is prevented from deteriorating. The methods for detecting biopolymers by trapping target biopolymers to the substrate side, comprise the steps of: 1) placing target biopolymers, with probe biopolymers and beads that are identified by antibodies or address probe peptides or biopolymer address linkers attached to their surface, in a solution; 2) hybridizing the target biopolymers with the probe biopolymers; and 3) identifying the address linkers bound to a substrate by the address probe peptide or biopolymers or polyclonal antibody molecules through antigen-antibody interactions. The methods for immobilizing antibodies comprise the steps of: 1) applying an amide group-containing gel embedded with antibody-binding molecules on a substrate of an insoluble substance in two or three dimensions; and 2) attaching the base of the antibody molecules to antibody-binding molecules.
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