Methods for identifying or screening for agents that modulate 17beta-HSD3

摘要

Methods for determining whether a test agent inhibits a 17beta-HSD3, said methods comprising: obtaining a recombinant host cell that expresses said 17beta-HSD3; obtaining a reaction mixture comprising said 17beta-HSD3, androstenedione, and said test agent; measuring the amount of testosterone in said reaction mixture by a scintillation proximity assay (SPA) using SPA beads conjugated with a testosterone-specific antibody, wherein when the amount of testosterone is lower in the presence of a test agent than in the absence of the test agent, the test agent is identified as an inhibitor of said 17beta-HSD3.