Melanogenesis-modulating macrolides assessed by mammalian cell based activity assays

摘要

The present invention relates to a compound having activity in a cell-based tyrosinase assay, obtainable or obtained by (a) culturing Streptomyces hygroscopicus subsp. hygroscopicus (DSMZ deposit number DSM 40578); (b) separating the medium containing the compound from the bacterial cells; (c) solid phase extraction of said medium, thereby obtaining a crude extract; (d) performing gel filtration of said crude extract; (e) taking a gel filtration fraction exhibiting activity in said assay; (f) performing HPLC of said fraction exhibiting activity in said assay; and (g) taking one or more HPLC fractions exhibiting activity in said assay, thereby obtaining said compound. The present invention also relates to methods of cosmetic treatment comprising the administration of a compound represented by the general formula (I) <CHEM> and to uses of said compound for the preparation of a cosmetic composition. Preferably, said use is a use wherein the application of the cosmetic composition modulates, or, more preferably, brings about the pigmentation of the body part to which said cosmetic composition is applied to. The present invention also relates to methods for identifying modulators of melanogenesis.