| 摘要 |
New gene (I) encodes 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS) that contains at least the Thr102Ile mutation. Also new are: (1) EPSPS mutant protein (II) with this mutation; (2) chimeric gene contg. (I) plus heterologous 5' and 3' regulatory elements; (3) vectors for plant transformation contg. at least one chimeric gene as in (2); and (4) plant cells (and plants derived from them) contg. the chimeric gene. The gene is pref. of bacterial or plant origin, in particular it is from Salmonella typhimurium or maize. The gene may further comprise at least one additional mutation, esp. resulting in the substitution Pro106Ser or Gly101Ala. The chimeric gene includes a promoter from a plant virus or plant, e.g. alpha -tubulin, histone, etc. Opt. it also includes a sequence encoding a transit peptide. |
