| 摘要 |
FIELD: molecular biology, medicinal industry. ^ SUBSTANCE: invention relates to a method for preparing recombinant double-stranded enzyme urokinase (ds-uAP). Method involves culturing cells CHO-Meissi subjected for genetic manipulations that are transfected stably with cDNA encoding pre-prourokinase in culturing medium containing alkanoic acids or their derivatives at temperature from 30°C to 37°C and the following separation the prepared ds-uAP for low-molecular and high-molecular forms. Also, invention involves recombinant ds-uAP, high-molecular ds-uAP and low-molecular ds-uAP in different stages of their preparing. Advantage of proposed method involves the development of method for preparing recombinant forms of mature double-stranded protein ds-uAP and its low-molecular and high-molecular forms. ^ EFFECT: improved preparing method. ^ 19 cl, 4 tbl, 5 ex |
