IMMUNOREACTION MEASUREMENT METHOD

摘要

A method of measuring an antigen or an antibody which is a subject to be measured contained in a sample. This method comprises mixing the sample with at least one compound selected from the group consisting of dicarboxylic acids having hydroxyl group, dicarboxylic acids having double bond, linear dicarboxylic acids represented by HOOC(CH2)nCOOH (wherein n is an integer) and salts thereof and an antibody or an antigen binding specifically to the subject to be measured as described above to give an acidic liquid reaction mixture, and then detecting an antigen-antibody complex formed by the antigen-antibody reaction between the subject to be measured and the substance binding specifically thereto in the liquid reaction mixture. Thus, measurement data can be improved and the restriction on the measurement range imposed by a zone phenomenon occurring in the region having the antigen in excess can be relieved.