Verfahren zur Herstellung von natürlich gefalteten Proteinen durch Co-Sekretion von molekularen Chaperonen

摘要

Preparing a naturally folded eukaryotic polypeptide (I) that contains two or more disulfide-bridged Cys residues by culturing prokaryotic cells that contain an expression vector for (I) including a prokaryotic signal sequence at its N-terminus and a nucleic acid (II) that secretes a chaperone protein (III) into the periplasm. (I) is secreted into the periplasm or medium; the signal peptide is then cleaved and (I) isolated from the periplasm or medium.