| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for crystallizing a protein, by which a high-quality protein crystal suitable for X-ray analysis, or the like, can be obtained. SOLUTION: This method for crystallizing the protein comprises a step (a) of adding a protein-containing sample to a gelated polymer keeping a protein crystallizing agent to make a protein solution formed by adding the protein-containing sample contact with the protein crystallizing agent and a step (b) of furthermore stratifying an oil phase on the protein-containing sample. The protein crystallizing agent is migrated slowly to the portion of the protein-containing sample from the gelated polymer and the protein-containing sample is migrated slowly to the gelated polymer keeping the protein crystallizing agent to cause a crystallization reaction little by little. Simultaneously, moisture is migrated to the oil phase to concentrate the protein solution gradually. Since the interaction, which is a crystallizing mechanism, between the protein and the surrounding environment (such as the presence of the protein crystallizing agent, the protein concentration and the pH) is advanced gradually by these two effects from two directions, the generation of a high-quality crystal nucleus can be promoted in a short time and the high-quality protein crystal can be obtained. COPYRIGHT: (C)2005,JPO&NCIPI
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