Fluorescently labeled thiol-containing trapping agent for the quantitation and identification of reactive metabolites in vitro

摘要

The present application relates to a sensitive and quantitative method using dansylated glutathione as a trapping agent for the detection of reactive metabolites in the field of drug discovery. The fluorescent tag attached to the dansylated glutathione does not impede the ability of glutathione to react with reactive metabolites.