PROBE FOR DETECTING NUCLEAR RECEPTOR AGONIST OR ANTAGONIST AND METHOD OF SCREENING AGONIST OR ANTAGONIST TO NUCLEAR RECEPTOR WITH THE USE OF THE SAME

摘要

A probe for detecting an agonist or an antagonist to a nuclear receptor characterized in that at least a ligand-recognition site containing a ligand-binding domain of the nuclear receptor is ligated to a binding-responsive site containing a peptide chain specifically binding to a coactivator-binding domain in the ligand-binding domain of the nuclear receptor via a flexible linker, and two marker sites, which allow the detection of the approach to each other, are ligated to the respective ends of the fused structure of [ligand-recognition site/linker/binding-responsive site].