Methods and compositions for detecting nucleic acids

摘要

A reporter molecule for detecting a nucleic acid is disclosed. The molecule comprises an enzyme having a k_cat of at least about 200 sec^-1, a reversible inhibitor of the enzyme inhibitorily engaging the enzyme, and a nucleobase polymer extending between the enzyme and the inhibitor. The polymer interferes with the engagement between the inhibitor and the enzyme when the nucleic acid contacts the polymer. Methods of making and methods of using the reporter molecule are also disclosed.