发明名称 |
PROCESS FOR PRODUCING PROTEIN IN CELL-FREE PROTEIN SYNTHESIS SYSTEM USING THIOREDOXIN FUSED PROTEIN EXPRESSION VECTOR |
摘要 |
A fusion protein of thioredoxin with a target protein is expressed in a cell-free protein synthesis system to thereby synthesize at least a part of the fusion protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In particular, a membrane protein such a G protein-coupled receptor can be produced in a state being highly soluble and capable of forming a biologically active three dimensional structure. Also, provided a recombinant vector for producing such a membrane protein in a cell-free protein synthesis system. |
申请公布号 |
EP1507003(A4) |
申请公布日期 |
2005.08.24 |
申请号 |
EP20030730531 |
申请日期 |
2003.05.21 |
申请人 |
RIKEN |
发明人 |
SHIROUZU, MIKAKO;ISHIHARA, GOUSHI;SAEKI, MIHORO;GOTO, MIE;TAJIMA, KAORI;KIGAWA, TAKANORI;YOKOYAMA, SHIGEYUKI |
分类号 |
C07K14/705;C12N9/02;C12N15/62;C12P21/00;C12P21/02 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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