| 摘要 |
PROBLEM TO BE SOLVED: To provide a minicell display method which has significant advantages for screening peptide libraries for candidates that can bind and effectively modulate a particular biological process. SOLUTION: The method, based on the small and anucleate minicell, increases versatility in generating unique sequences to be screened and increases the size of the peptides to be screened. In vitro mutagenesis, at the level of protein synthesis, as well as DNA replication, increases versatility of the library to be screened and therefore substantially increases the number of potential peptides that can modulate a particular biological response or mechanism. COPYRIGHT: (C)2005,JPO&NCIPI |
