发明名称 |
Chemically modified proteins with a carbohydrate moiety |
摘要 |
The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine in a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also, a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins.
|
申请公布号 |
US6930180(B2) |
申请公布日期 |
2005.08.16 |
申请号 |
US20020062970 |
申请日期 |
2002.02.01 |
申请人 |
GENENCOR INTERNATIONAL, INC. |
发明人 |
JONES J. BRYAN;DAVIS BENJAMIN G. |
分类号 |
C12N15/09;C07K14/00;C12N9/54;C12N9/96;C12Q1/25;C12Q1/37;(IPC1-7):C07G3/00 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|