Method and system for reducing total sample complexity

摘要

The present invention relates to a method for reducing total sample complexity in native or digested biological sample(s), before analysis thereof by mass spectrometry, comprising the following steps: a) selecting a fraction from the entire native or digested biological sample(s) on the basis of pi-value, said fraction comprising native or digested sample representing a subset of or the entire substance population in the sample; b) separating native or digested sample substances from each other; and c) analysing said substances by mass spectrometry. The invention also relates to a system for reducing total sample complexity in the above method, comprising a high capacity charge-selective column coupled to a MDLC work flow path comprising a cation exchange column and a RPC column. The system is followed by a MS/MS instrument.