发明名称 |
Homogeneous assay methods |
摘要 |
The present invention provides a method of assaying for kinase activity, comprising contacting a fluorescently labeled phosphorylatable peptide substrate with an ATP analog in the presence of a kinase enzyme to yield a first product; contacting the first product with a reactant that comprises a biotin derivative to yield a second product; contacting the second product with a biotin-binding protein; and detecting a difference in a fluorescence polarization level from the second product as compared to a fluorescence polarization of the peptide substrate.
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申请公布号 |
US2005176071(A1) |
申请公布日期 |
2005.08.11 |
申请号 |
US20040855657 |
申请日期 |
2004.05.27 |
申请人 |
CALIPER LIFE SCIENCES, INC. |
发明人 |
NIKIFOROV THEO T.;JEONG SANG |
分类号 |
C12Q1/48;G01N33/542;G01N33/543;(IPC1-7):G01N33/53 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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